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(A) Total Wg distribution after dlp inactivation ( left), schlank knockdown ( right ) or both ( middle ) in the vg-Gal4 domain. (B) Total Wg (from <t>SNAP-Wg</t> labelled <t>with</t> <t>SNAP-TMR)</t> and Dlp (from immunofluorescence) after schlank knockdown under the control of hh-Gal4 . Anti-Ci marks the anterior compartment. Both WgLVs and Wg punctae appear in the schlank knockdown compartment while only WgLVs are seen in the control compartment. Dlp was detectable in some but not all WgLVs and in some but not all the aggregates.
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(A) Total Wg distribution after dlp inactivation ( left), schlank knockdown ( right ) or both ( middle ) in the vg-Gal4 domain. (B) Total Wg (from <t>SNAP-Wg</t> labelled <t>with</t> <t>SNAP-TMR)</t> and Dlp (from immunofluorescence) after schlank knockdown under the control of hh-Gal4 . Anti-Ci marks the anterior compartment. Both WgLVs and Wg punctae appear in the schlank knockdown compartment while only WgLVs are seen in the control compartment. Dlp was detectable in some but not all WgLVs and in some but not all the aggregates.
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(A) Total Wg distribution after dlp inactivation ( left), schlank knockdown ( right ) or both ( middle ) in the vg-Gal4 domain. (B) Total Wg (from <t>SNAP-Wg</t> labelled <t>with</t> <t>SNAP-TMR)</t> and Dlp (from immunofluorescence) after schlank knockdown under the control of hh-Gal4 . Anti-Ci marks the anterior compartment. Both WgLVs and Wg punctae appear in the schlank knockdown compartment while only WgLVs are seen in the control compartment. Dlp was detectable in some but not all WgLVs and in some but not all the aggregates.
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(A) Total Wg distribution after dlp inactivation ( left), schlank knockdown ( right ) or both ( middle ) in the vg-Gal4 domain. (B) Total Wg (from <t>SNAP-Wg</t> labelled <t>with</t> <t>SNAP-TMR)</t> and Dlp (from immunofluorescence) after schlank knockdown under the control of hh-Gal4 . Anti-Ci marks the anterior compartment. Both WgLVs and Wg punctae appear in the schlank knockdown compartment while only WgLVs are seen in the control compartment. Dlp was detectable in some but not all WgLVs and in some but not all the aggregates.
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(A) Total Wg distribution after dlp inactivation ( left), schlank knockdown ( right ) or both ( middle ) in the vg-Gal4 domain. (B) Total Wg (from <t>SNAP-Wg</t> labelled <t>with</t> <t>SNAP-TMR)</t> and Dlp (from immunofluorescence) after schlank knockdown under the control of hh-Gal4 . Anti-Ci marks the anterior compartment. Both WgLVs and Wg punctae appear in the schlank knockdown compartment while only WgLVs are seen in the control compartment. Dlp was detectable in some but not all WgLVs and in some but not all the aggregates.
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(A) Total Wg distribution after dlp inactivation ( left), schlank knockdown ( right ) or both ( middle ) in the vg-Gal4 domain. (B) Total Wg (from <t>SNAP-Wg</t> labelled <t>with</t> <t>SNAP-TMR)</t> and Dlp (from immunofluorescence) after schlank knockdown under the control of hh-Gal4 . Anti-Ci marks the anterior compartment. Both WgLVs and Wg punctae appear in the schlank knockdown compartment while only WgLVs are seen in the control compartment. Dlp was detectable in some but not all WgLVs and in some but not all the aggregates.
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Image Search Results


(A) Total Wg distribution after dlp inactivation ( left), schlank knockdown ( right ) or both ( middle ) in the vg-Gal4 domain. (B) Total Wg (from SNAP-Wg labelled with SNAP-TMR) and Dlp (from immunofluorescence) after schlank knockdown under the control of hh-Gal4 . Anti-Ci marks the anterior compartment. Both WgLVs and Wg punctae appear in the schlank knockdown compartment while only WgLVs are seen in the control compartment. Dlp was detectable in some but not all WgLVs and in some but not all the aggregates.

Journal: bioRxiv

Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells

doi: 10.64898/2026.02.12.705585

Figure Lengend Snippet: (A) Total Wg distribution after dlp inactivation ( left), schlank knockdown ( right ) or both ( middle ) in the vg-Gal4 domain. (B) Total Wg (from SNAP-Wg labelled with SNAP-TMR) and Dlp (from immunofluorescence) after schlank knockdown under the control of hh-Gal4 . Anti-Ci marks the anterior compartment. Both WgLVs and Wg punctae appear in the schlank knockdown compartment while only WgLVs are seen in the control compartment. Dlp was detectable in some but not all WgLVs and in some but not all the aggregates.

Article Snippet: SNAP labelling reagents used: SNAP-surface488 (NEB, S9124S), SNAPCell-TMR-Star (NEB, S9105S) and SNAP-surface649 (NEB, S9159S).

Techniques: Knockdown, Immunofluorescence, Control